Resumen:
Native chemical ligation (NCL) employing the
N-methylbenzimidazolinone (MeNbz) linker readily provided
the linear precursor of a 16-mer peptide that is difficult to
obtain by stepwise solid-phase peptide synthesis. NCL and the
workup conditions were improved toward a protocol that
allows for quantitative removal of the 4-hydroxymercapto-
phenol additive and subsequent formation of the disulfide
bridge in the NCL cocktail by oxidation in air, tolerated by the
presence of tris(hydroxypropyl)phosphine.