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Título : Epigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potential
Autor : Pila Quinga, Liliana Alexandra
Fraga, Hugo
do Nascimento Vieira, Leila
Guerra, Miguel Pedro
Palabras clave : Epigenetics
Fecha de publicación : 2017
Editorial : Kluwer Academic Publishers
Citación : Pila Quinga, L. A., Pacheco de Freitas Fraga, H., do Nascimento Vieira, L., & Guerra, M. P. (2017). Epigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potential. Plant Cell, Tissue and Organ Culture, 131(2), 295–305. doi.org/10.1007/s11240-017-1284-6
Resumen : In Theobroma cacao L., declined embryogenic potential was observed in regenerated somatic embryos from long-term secondary somatic embryogenesis (SE). In order to explore the relationship between DNA methylation and the long-term secondary SE, the embryogenic potential and global DNA methylation levels in young (12 months-old), aged (36 months-old) and extra somatic embryogenesis (39 months-old) subjected to different 5-Azacytidine (5-azaC) treatments were comparatively assessed. Global DNA methylation levels increased in aged somatic embryos with long-term in vitro culture, but 5-azaC-supplemented treatments resulted in unaltered levels. In addition, DNA methylation pattern during SE was not affected by 5-azaC. DNA methylation increased during SE expression. Interestingly, the extra SE induction showed that aged somatic embryos can recovery the embryogenic potential in treatment supplemented with 5-azaC at specific concentration. The outcome of this study suggested that the long-term SE in cacao induced the decline on embryogenic potential, which can be reversible trough 5-azaC supplementation. Besides, increased DNA methylation levels might be a response to the stress conditions that plant cells were exposed to during SE.
URI : http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/158
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