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dc.contributor.authorPila Quinga, Liliana Alexandra-
dc.contributor.authorFraga, Hugo-
dc.contributor.authordo Nascimento Vieira, Leila-
dc.contributor.authorGuerra, Miguel Pedro-
dc.identifier.citationPila Quinga, L. A., Pacheco de Freitas Fraga, H., do Nascimento Vieira, L., & Guerra, M. P. (2017). Epigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potential. Plant Cell, Tissue and Organ Culture, 131(2), 295–305. doi.org/10.1007/s11240-017-1284-6es
dc.identifier.otherDOI: 10.1007/s11240-017-1284-6-
dc.description.abstractIn Theobroma cacao L., declined embryogenic potential was observed in regenerated somatic embryos from long-term secondary somatic embryogenesis (SE). In order to explore the relationship between DNA methylation and the long-term secondary SE, the embryogenic potential and global DNA methylation levels in young (12 months-old), aged (36 months-old) and extra somatic embryogenesis (39 months-old) subjected to different 5-Azacytidine (5-azaC) treatments were comparatively assessed. Global DNA methylation levels increased in aged somatic embryos with long-term in vitro culture, but 5-azaC-supplemented treatments resulted in unaltered levels. In addition, DNA methylation pattern during SE was not affected by 5-azaC. DNA methylation increased during SE expression. Interestingly, the extra SE induction showed that aged somatic embryos can recovery the embryogenic potential in treatment supplemented with 5-azaC at specific concentration. The outcome of this study suggested that the long-term SE in cacao induced the decline on embryogenic potential, which can be reversible trough 5-azaC supplementation. Besides, increased DNA methylation levels might be a response to the stress conditions that plant cells were exposed to during SE.es
dc.publisherKluwer Academic Publisherses
dc.relation.ispartofseriesPRODUCCIÓN CIENTÍFICA-ARTÍCULOS;A-IKIAM-000096-
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América*
dc.titleEpigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potentiales
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