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Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins

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dc.contributor.author Bermúdez Puga, Sebastián
dc.contributor.author Dias, Meriellen
dc.contributor.author Lima Reis, Iara
dc.contributor.author Freire de Oliveira, Taciana
dc.contributor.author Yokomizo de Almeida, Sonia Regina
dc.contributor.author Mendes, Maria Anita
dc.contributor.author Moore, Simon J.
dc.contributor.author de Almeida, José R.
dc.contributor.author Proaño Bolaños, Carolina
dc.contributor.author de Souza Oliveira, Ricardo Pinheiro
dc.date.accessioned 2024-11-06T17:15:03Z
dc.date.available 2024-11-06T17:15:03Z
dc.date.issued 2024
dc.identifier.citation Bermúdez-Puga, S., Dias, M., Lima Reis, I., Freire de Oliveira, T., Yokomizo de Almeida, S. R., Mendes, M. A., Moore, S. J., Almeida, J. R., Proaño-Bolaños, C., & Pinheiro de Souza Oliveira, R. (2024). Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins. Biochimie, 225, 168-175. https://doi.org/10.1016/j.biochi.2024.05.022 es
dc.identifier.issn 1638-6183
dc.identifier.uri https://doi.org/10.1016/j.biochi.2024.05.022
dc.identifier.uri http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/801
dc.description.abstract Listeria monocytogenes is a human opportunistic foodborne pathogen that produces life-threatening infections with a high mortality rate. The control of Listeria in the food production environment and effective clinical management of human listeriosis are challenging due to the emergence of antibiotic resistance. Hence we evaluate the in vitro anti-Listeria activity of two synthetic cruzioseptins reproducing their natural sequences CZS-9, and CZS-12, and one engineered sequence based on CZS-1, named [K4K15]CZS-1. The assessment of the in vitro potential of cruzioseptins, highlighted the promising antibacterial effect of [K4K15]CZS-1 in very low concentrations (0.91 μM) and its thermal stability at high-temperature conditions, is compatible with the food industry. Microscopic and metabolomic analyses suggest cruzioseptin induces anti-Listeria bioactivity through membrane disruption and changes in the intracellular metabolome. We also report that [K4K15]CZS-1 is not resistant to peptidases/proteases emphasizing a key advantage for their use as a food preservative. However, there is a need for further structural and functional optimisations for the potential clinical application as an antibiotic. In conclusion, [K4K15]CZS-1 stand out as membrane-active peptides with the ability to induce shifts in the bacteria metabolome and inspire the development of strategies for the prevention of L. monocytogenes emergence and dissemination. es
dc.language.iso en es
dc.publisher Scopus es
dc.relation.ispartofseries PRODUCCIÓN CIENTÍFICA-ARTÍCULO CIENTÍFICO;A-IKIAM-000533
dc.subject Antibacterial es
dc.subject Listeria monocytogenes es
dc.subject Membrane lysis es
dc.subject Metabolomics es
dc.subject Skin secretion-derived peptides es
dc.title Microscopic and metabolomics analysis of the anti-Listeria activity of natural and engineered cruzioseptins es
dc.type Article es


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