Resumen:
Batrachochytrium dendrobatidis (Bd) is a lethal fungal species that parasitizes vertebrates
and is associated with the worldwide decline of amphibian populations. The development of
sensitive, rapid detection methods, particularly DNA-based techniques, is critical for effective management strategies. This study evaluates the efficacy of DNA extraction and a portable PCR device in a mountable field laboratory setup for detecting Bd near the habitats of
three critically endangered Atelopus toad species in Ecuador. We collected skin swabs from
Atelopus balios, A. nanay, and A. bomolochos, and environmental DNA (eDNA) samples
from streams in Andean and coastal regions of Ecuador. For eDNA, a comparison was
made with duplicates of the samples that were processed in the field and in a standard university laboratory. Our findings revealed Bd detection in eDNA and swabs from 6 of 12
water samples and 10 of 12 amphibian swab samples. The eDNA results obtained in the
field laboratory were concordant with those obtained under campus laboratory conditions.
These findings highlight the potential of field DNA-based monitoring techniques for detecting
Bd in amphibian populations and their aquatic habitats, particularly in remote areas. Furthermore, this research aligns with the National Action Plan for the Conservation of Ecuadorian
Amphibians and contributes to the global effort to control this invasive and deadly fungus
