Resumen:
Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in
the structural diversification of triterpenoids. To perform site-specific oxidations
of the triterpene scaffold, CYPs require electrons transferred by NADPH cytochrome P450 reductase (CPR), which is classified into two main classes,
class I and class II, based on their structural difference. Lotus japonicus is a
triterpenoids-producing model legume with one CPR class I gene (LjCPR1) and a
minimum of two CPR class II genes (LjCPR2-1 and LjCPR2-2). CPR classes I and II
from different plants have been reported to be involved in different metabolic
pathways. By performing gene expression analyses of L. japonicus hairy root
culture treated with methyl jasmonate (MeJA), this study revealed that LjCPR1,
CYP716A51, and LUS were down-regulated which resulted in no change in
betulinic acid and lupeol content. In contrast, LjCPR2s, bAS, CYP93E1, and
CYP72A61 were significantly upregulated by MeJA treatment, followed by a
significant increase of the precursors for soyasaponins, i.e. b-amyrin, 24-OH b amyrin, and sophoradiol content. Triterpenoids profile analysis of LORE1
insertion and hairy root mutants showed that the loss of the Ljcpr2-1 gene
significantly reduced soyasaponins precursors but not in Ljcpr1 mutants.
However, Ljcpr1 and Ljcpr2-1 mutants showed a significant reduction in lupeol
and oleanolic, ursolic, and betulinic acid contents. Furthermore, LjCPR1, but not
LjCPR2, was crucial for seed development, supporting the previous notion that
CPR class I might support plant basal metabolism. This study suggests that CPR
classes I and II play different roles in L. japonicus triterpenoid biosynthesis
