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Título : Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae)
Autor : Proaño Bolaños, Carolina
Blasco Zúñiga, Ailín
de Almeida, José R.
Wang, Lei
Llumiquinga, Miguel Angel
Rivera, Miryan
Zhou, Mei
Chen, Tianbao
Shaw, Chris
Palabras clave : Dermaseptins
Frog skin secretion
Tandem mass spectrometry
Agalychnis spurrelli
Fecha de publicación : 2019
Editorial : Multidisciplinary Digital Publishing Institute (MDPI)
Citación : Proaño Bolaños, Carolina, Blasco Zúñiga A, Almeida JR, et al. Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae). Biomolecules. 2019;9(11):667. Published 2019 Oct 30. doi:10.3390/biom9110667
Resumen : Frog skin secretions contain medically-valuable molecules, which are useful for the discovery of new biopharmaceuticals. The peptide profile of the skin secretion of Agalychnis spurrelli has not been investigated; therefore, the structural and biological characterization of its compounds signify an inestimable opportunity to acquire new biologically-active chemical scaffolds. In this work, skin secretion from this amphibian was analysed by molecular cloning and tandem mass spectrometry. Although the extent of this work was not exhaustive, eleven skin secretion peptides belonging to five peptide families were identified. Among these, we report the occurrence of two phyllokinins, and one medusin-SP which were previously reported in other related species. In addition, eight novel peptides were identified, including four dermaseptins, DRS-SP2 to DRS-SP5, one phylloseptin-SP1, and three orphan peptides. Phylloseptin-SP1 and dermaseptins-SP2 were identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. Among the antimicrobial peptides, dermaseptin-SP2 was the most potent, inhibiting Escherichia coli, Staphylococcus aureus, and ORSA with a minimum inhibitory concentration (MIC) of 2.68 μM, and Candida albicans with an MIC of 10.71 μM, without haemolytic effects. The peptides described in this study represent but a superficial glance at the considerable structural diversity of bioactive peptides produced in the skin secretion of A. spurrelli
URI : http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/344
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