Por favor, use este identificador para citar o enlazar este ítem: http://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/309
Registro completo de metadatos
Campo DC Valor Lengua/Idioma
dc.contributor.authorSrisawat, Pisanee-
dc.contributor.authorFukushima, Ery Odette-
dc.contributor.authorYasumoto, Shuhei-
dc.contributor.authorRobertlee, Jekson-
dc.contributor.authorSuzuki, Hideyuki-
dc.contributor.authorSeki, Hikaru-
dc.date.accessioned2019-08-19T13:28:35Z-
dc.date.available2019-08-19T13:28:35Z-
dc.date.issued2019-
dc.identifier.citationSrisawant, Pisanee, Fukushima, Ery Odette, Robertle, Jekson, Suzuki, Hedeyuki, Seki, Hikaru,& Muranaka, Toshiya (2019). Identification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α‐amyrin‐producing activity. New Phytologist, 8/228. DOI: 10.1111/nph.16013es
dc.identifier.urihttp://repositorio.ikiam.edu.ec/jspui/handle/RD_IKIAM/309-
dc.identifier.urihttps://doi.org/10.1111/nph.16013-
dc.description.abstractTriterpenoids are widely distributed among plants of the legume family. However, most studies have focused on triterpenoids and their biosynthetic enzymes in model legumes. We evaluated the triterpenoid aglycones profile of the medicinal legume tree Bauhinia forficata by gas chromatography-mass spectrometry. Through transcriptome analyses, homology-based cloning, and heterologous expression, we discovered four oxidosqualene cyclases (OSCs) which are responsible for the diversity of triterpenols in B. forficata. We also investigated the effects of the unique motif TLCYCR on α-amyrin synthase activity. B. forficata highly accumulated α-amyrin. We discovered an OSC with a preponderant α-amyrin-producing activity, which accounted for at least 95% of the total triterpenols. We also discovered three other functional OSCs (BfOSC1, BfOSC2, and BfOSC4) that produce β-amyrin, germanicol, and cycloartenol. Furthermore, by replacing the unique motif TLCYCR from BfOSC3 with the MWCYCR motif, we altered the function of BfOSC3 such that it no longer produced α-amyrin. Our results provide new insights into OSC cyclization, which is responsible for the diversity of triterpenoid metabolites in B. forficata, a non-model legume plant.es
dc.language.isoenes
dc.publisherBlackwell Publishing Inc.es
dc.rightsopenAccesses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectBauhinia forficataes
dc.subjectNon‐model legumees
dc.subjectOxidosqualene cyclasees
dc.subjectTriterpenoides
dc.subjectα‐amyrines
dc.titleIdentification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α-amyrin-producing activityes
dc.typeArticlees
Aparece en las colecciones: ARTÍCULOS CIENTÍFICOS

Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
A-IKIAM-000212.pdfIdentification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering pre ponderant a-amyrin-producing activit2,9 MBAdobe PDFVista previa
Visualizar/Abrir


Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons Creative Commons